19F核磁共振定量法测定含氟漱口液中氟化钠的含量
投稿时间:2019-04-12  修订日期:2019-07-20  点此下载全文
引用本文:华俊杰,朱海菲,李育.19F核磁共振定量法测定含氟漱口液中氟化钠的含量[J].药学实践杂志,2019,37(6):518~520
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作者单位E-mail
华俊杰 海军军医大学药学院 上海 200433  
朱海菲 海军军医大学药学院 上海 200433  
李育 海军军医大学药学院 上海 200433 fishleesmmu@126.com 
中文摘要:目的 建立19F核磁共振技术测定含氟漱口液中氟化钠含量的方法。方法 采用19F核磁共振技术,以三氟乙酸作为内标物,使用300MHz核磁共振波谱仪,在25℃下采集样品的19F核磁共振谱,建立内标标准曲线,用于氟化钠定量分析。结果 氟化钠在0.1~0.8 mg/ml范围内,线性关系良好,回归方程为A=1.1392C-0.0013,r=0.99995;低、中、高3个浓度的平均回收率分别为99.36%、99.31%、99.65%,RSD分别为0.51%、0.35%、0.54%,含氟漱口液中氟化钠的平均含量为99.30%。结论 研究建立的19F核磁共振方法准确度、精密度较好,能够快速、准确地测定含氟漱口液中氟化钠的含量。
中文关键词:19F-NMR  氟化钠  含量测定  定量核磁共振
 
Determination of sodium fluoride in fluoride-containing mouthwash by 19F-NMR
Abstract:Objective To establish a method for the determination of sodium fluoride in fluoride-containing mouthwash by 19F-NMR technique.Methods The 19F-NMR technique was used to establish the relevant standard curve with trifluoroacetic acid as the internal standard.The 19F-NMR spectrum of the sample was collected at 25℃ using a 300 MHz NMR spectrometer.Results The linear relationship of sodium fluoride was good in the range of 0.1-0.8 mg/ml.The regression equation was A=1.1392C-0.0013,r=0.99995.The average recovery of low,medium and high concentration levels were 99.36%,99.31.% and 99.65%,respectively,and RSD was 0.51%,0.35% and 0.54% respectively.The average content of sodium fluoride in the fluoride mouthwash was 99.30%.Conclusion The method was accurate and precise which could be used to determine the sodium fluoride in fluoride-containing mouthwash.
keywords:19F-NMR  sodium fluoride  determination of content  standard curve
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