基于UPLC-Q-TOF-MS技术鉴定防风在大鼠体内的入血成分及代谢产物
投稿时间:2019-06-15  修订日期:2019-11-05  点此下载全文
引用本文:李悦悦,章斌,原永芳.基于UPLC-Q-TOF-MS技术鉴定防风在大鼠体内的入血成分及代谢产物[J].药学实践杂志,2019,37(6):512~517
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作者单位E-mail
李悦悦 上海交通大学医学院附属第九人民医院药剂科, 上海 200011  
章斌 上海交通大学医学院附属第九人民医院药剂科, 上海 200011  
原永芳 上海交通大学医学院附属第九人民医院药剂科, 上海 200011 nmxyyf@126.com 
中文摘要:目的 研究防风Saposhnikovia divaricata(Turcz.)Schischk.水煎液在大鼠体内的入血成分及代谢产物。方法 应用超高效液相色谱串联飞行时间质谱(UPLC-Q-TOF-MS)技术鉴定防风入血成分及代谢产物。采用Eclipse Plus C18色谱柱(150mm×2.1mm,1.8μm),流动相为0.1%甲酸水溶液(A)-0.1%甲酸乙腈(B),梯度洗脱,流速0.3 ml/min,进样量3 μl。结果 建立了大鼠给药后血浆样品的分析测定方法,并且与空白血浆、防风水煎液和对照品的保留时间及质谱碎片离子信息对比,共鉴定了大鼠血浆中21个来源于防风的化合物,包括10个入血原型成分和11个代谢产物。结论 防风主要成分在大鼠体内的代谢途径主要包括羟化、去甲基化、葡萄糖醛酸化等。所建立的方法简单可靠,为揭示防风的药效物质基础提供研究依据。
中文关键词:防风  超高效液相色谱  串联飞行时间质谱  入血成分  代谢产物  升麻素
 
Identification of components and metabolites of Saposhnikoviae Radix decoction in rats' plasma by UPLC-Q-TOF-MS
Abstract:Objective To study the prototype components and metabolites of Saposhnikoviae Radixin in rats' plasma.Methods UPLC-Q-TOF-MS method was used to identify the components and metabolites of rat plasma.The Eclipse Plus C18 column(150 mm×2.1 mm,1.8 μm,Agilent)was used.The mobile phase was 0.1% acetic acid solution (A)-0.1% acetic acidacetonitrile (B).The gradient elution was used.The flowing rate was 0.3 ml/min.The injection volume was 3 μl.Results An analytical method for the determination of metabolites of Saposhnikoviae Radix in plasma samples after oral administration.A total of 21 compounds,including 10 prototype components and 11 metabolites,were identified by comparison of their retention time,ion fragmentation information with that of the control plasma,herb extract,and reference compounds.Conclusion The metabolic pathway of the main components of Saposhnikoviae Radixin in rats mainly include hydroxylation,demethylation and glucuronidation,etc.This established method is simple and reliable and provide research basis for revealing the bioactive components of Saposhnikoviae Radix.
keywords:Saposhnikoviae Radix  UPLC-Q-TOF-MS  ingredients in plasma  metabolites  cimifugin
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