HPLC法同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量
投稿时间:2019-08-20  修订日期:2019-11-04  点此下载全文
引用本文:韩凌,王新霞,吕磊,张静如,阚月一,鲁莹,傅翔.HPLC法同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量[J].药学实践杂志,2019,37(6):508~511
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作者单位E-mail
韩凌 上海控江医院药剂科, 上海 200093
海军军医大学药学院, 上海 200433 
 
王新霞 上海东方肝胆外科医院药材科, 上海 200438  
吕磊 上海东方肝胆外科医院药材科, 上海 200438  
张静如 海军军医大学药学院, 上海 200433  
阚月一 上海东方肝胆外科医院药材科, 上海 200438  
鲁莹 海军军医大学药学院, 上海 200433  
傅翔 上海控江医院药剂科, 上海 200093 15000611618@139.com 
基金项目:国家科技重大专项资助项目(2018ZX09J18110-001-001)
中文摘要:目的 建立同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量的方法。方法 采用高效液相色谱法。色谱柱为Agilent Extend-C18(4.6 mm×150 mm,5 μm),流动相为甲醇-乙酸铵缓冲溶液(20 mmol/L,pH=7.5),梯度洗脱,甲醇比例随时间变化为:0~10 min、甲醇→60%;10~12 min、甲醇60%→92%;12~20 min、甲醇→92%,采集时间20 min,平衡时间10 min,流速1 ml/min,紫外检测波长218 nm,柱温25℃,进样量15 μl。结果 3种成分在30 min内基线分离,方法学验证表明,线性关系良好(r>0.9995),日内日间精密度均小于3.0%,回收率在90%~108%之间,稳定性和重复性RSD均<3.0%,符合方法学要求。按照新建立的方法测定了3个批次样品中3种成分的含量,结果符合要求。结论 本方法简便可靠,可为多效防护霜的质量控制提供依据,也为其质量标准研究奠定了基础。
中文关键词:多效防护霜  高效液相色谱  盐酸苯海拉明  甲氧基肉桂酸辛酯  水杨酸辛酯  含量测定
 
Simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream by HPLC
Abstract:Objective To establish a HPLC method for the simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream.Methods The HPLC condition was as follows.The column was Agilent Extend-C18 (4.6 mm×150 mm,5 μm).The mobile phase was methanol and ammonium acetate buffer solution (20 mmol/L,pH=7.5).Gradient elution.The methanol ratio varies with time as follows:0-10 min,methanol→60%; 10-12 min,methanol 60%→92%;12-20 min,methanol→92%.Acquisition time was 20 minutes.Liquid phase equilibrium time was 10 min.Flow rate was 1 ml/min.UV detection wavelength was 218 nm.Column temperature was 25℃.Injection volume was 15 μl.Results The three components were separated at baseline within 30 min.The methodological verification showed that the standard curves had good linearity (r>0.9995).The inter-day precision were less than 3.0%.The recovery was between 90% and 108%.The RSD% of stability and repeatability were also less than 3.0%.The results showed that the method could meet the requirements of method validation.The contents of the three components in three batches were determined according to the newly established method.Conclusion The method is simple and reliable,can provide a basis for the quality control of multi-effect protective cream,and laid a foundation for its quality standard research.
keywords:multi-effect protective cream  HPLC  diphenhydramine hydrochloride  octyl methoxycinnamate  octyl salicylate  content determination
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