单核细胞移动抑制因子对大鼠创伤性颅脑损伤保护作用研究
投稿时间:2019-07-23  修订日期:2019-10-08  点此下载全文
引用本文:丁华敏,秦春霞,马凯源,孙莉莉,章越凡,李铁军.单核细胞移动抑制因子对大鼠创伤性颅脑损伤保护作用研究[J].药学实践杂志,2019,37(6):498~502
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作者单位E-mail
丁华敏 上海市浦东新区浦南医院药剂科, 上海 200125  
秦春霞 上海市浦东新区浦南医院药剂科, 上海 200125  
马凯源 安徽中医药大学药学院, 安徽 合肥 230012  
孙莉莉 上海市浦东新区浦南医院药剂科, 上海 200125  
章越凡 上海市浦东新区浦南医院药剂科, 上海 200125  
李铁军 上海市浦东新区浦南医院药剂科, 上海 200125
安徽中医药大学药学院, 安徽 合肥 230012 
18930502906@163.com 
基金项目:上海市卫生和计划生育委员会科研课题(201640404);上海市浦东新区卫生系统重要薄弱学科建设资助(PWZbr2017-16)
中文摘要:目的 研究单核细胞移动抑制因子(MLIF)对大鼠颅脑创伤(TBI)的保护作用,并初步探讨其作用机制。方法 将大鼠随机分为5组,假手术组、模型组、MLIF低、中、高剂量组(0.33、1、3 mg/kg)。采用液压冲击法制备大鼠颅脑创伤模型,颅脑打击后30 min内完成首次尾静脉注射给药,每天给药1次,连续给药7 d。分别于给药后1、3、7 d取脑,检测大鼠脑含水量变化;采用试剂盒法检测血清超氧化物歧化酶(SOD)和丙二醛(MDA)水平;HE染色及尼氏染色观察脑组织病理改变。结果 液压打击致大鼠颅脑创伤24 h后,MLIF低、中、高剂量组大鼠脑含水量均显著低于模型组(P<0.01),MLIF中剂量组(1 mg/kg)效果最好。与模型组比较,创伤后1、3 d,MLIF(1mg/kg)组大鼠脑含水量显著降低(P<0.01),该组大鼠血清中SOD含量明显升高(P<0.05)和MDA含量显著降低(P<0.05)。HE染色和尼氏染色结果显示,模型组大鼠脑组织病理损伤明显,呈现明显水肿、细胞固缩等;MLIF(1mg/kg)组大鼠脑组织病理损伤得到改善,水肿程度减轻。结论 MLIF可以抑制脑损伤氧化应激及水肿反应,发挥颅脑损伤保护作用。
中文关键词:单核细胞移动抑制因子  颅脑损伤  脑水肿  大鼠
 
Protective effect of MLIF on traumatic brain injury in rats
Abstract:Objective To study the protective effect and explore the mechanism of monocyte locomotion inhibitor factor(MLIF) on traumatic brain injury (TBI) in rats.Methods The SD rats were randomly divided into five groups:sham operation group,model group and three MLIF groups (0.33 mg/kg,1 mg/kg,3 mg/kg).Rats were made model of brain injury by fluid percussion injury.The tail vein was administered within 30 min after TBI,once every 24 hours,and rats were administered for 7 days.Brain water content,serum levels of SOD and MDA,Hematoxylin-eosin staining and Nissl staining were used to detect the rats brain tissues.Results After rats traumatic brain injury for 24h,the brain water content in rats in three MLIF groups(0.33 mg/kg,1 mg/kg and 3 mg/kg)was significantly lower than that in model group(P<0.01),The effect of MLIF (1 mg/kg) group was the best.Compared to rats traumatic brain injury for 1d and 3d,brain water content in MLIF group (1 mg/kg) was significantly lower than that in model group (P<0.01).SOD content in serum in MLIF (1 mg/kg) group was significantly increased (P <0.01) and MDA content in serum in MLIF (1 mg/kg) group was significantly decreased (P<0.05).HE staining and Nissl staining showed that the pathological changes of brain tissue in model group were obvious,and which in MLIF group were improved in brain tissue.Conclusion MLIF could inhibit oxidative stress and edema reaction of brain injury,which had protective effect on traumatic brain injury.
keywords:MLIF  traumatic brain injury  brain edema  rats
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