联用超高效液相色谱-高分辨飞行时间质谱技术对扶正化瘀胶囊化学成分快速鉴别
投稿时间:2019-01-09  修订日期:2019-03-29  点此下载全文
引用本文:陶朝阳,邢心睿,陈思,曹奇,丁德英,朱臻宇.联用超高效液相色谱-高分辨飞行时间质谱技术对扶正化瘀胶囊化学成分快速鉴别[J].药学实践杂志,2019,37(5):406~415
摘要点击次数: 35
全文下载次数: 30
作者单位E-mail
陶朝阳 西藏军区总医院药物制剂研究中心, 西藏 拉萨 8500071  
邢心睿 海军军医大学药学院, 上海 200433  
陈思 解放军第210医院, 辽宁 大连 116000  
曹奇 海军军医大学药学院, 上海 200433  
丁德英 海军军医大学药学院, 上海 200433  
朱臻宇 海军军医大学药学院, 上海 200433 zzyzyfzhu@163.com 
基金项目:国家自然科学基金(81773683)
中文摘要:目的 运用超高效液相色谱-高分辨飞行时间质谱(UHPLC-TOF/MS)联用技术鉴别扶正化瘀胶囊中化学成分。方法 色谱分离采用ACQUITY UPLCHSS T3柱(2.1 mm×100 mm,1.8 μm);流动相0.1%甲酸水溶液(A)-0.1%甲酸乙腈溶液(B),梯度洗脱条件:0~3 min,2% B;3~18 min,2%~50% B;18~22 min,50%~95% B;22~25 min,95% B。平衡时间为10 min,流速为0.4 ml/min,分析时间为25 min。质谱定性采用电喷雾离子源,正/负离子模式扫描,采集范围100~1 100 m/z结果 正/负离子模式下,一次性鉴别出154个成分,其中,正、负离子模式下均有响应的有28个。结论 较全面地阐明了扶正化瘀胶囊的成分组成,为该复方的深入研究奠定了基础。
中文关键词:扶正化瘀胶囊  鉴别  化学成分
 
Rapid identification of chemical constituents of Fuzheng Huayu capsules by UHPLC-TOF/MS
Abstract:Objective To identify the chemical constituents in Fuzheng Huayu capsule by ultra performance liquid chromatography-high resolution time of flight mass spectrometry(UHPLC-TOF/MS).Methods ACQUITY UPLCHSS T3(2.1mm×100mm,1.8μm) was used to chromatographic separation; mobile phase 0.1% formic acid aqueous solution(A) -0.1% formic acid acetonitrile solution(B),gradient elution conditions:0-3min,2% B; 3-18 min,2%-50% B; 18-22 min,50%-95% B; 22-25 min,95% B.The equilibration time was 10 min,the flow rate was 0.40 ml/min,and the analysis time was 25 min.Mass spectrometry was performed by an electrospray ion source using a positive-negative ion mode scan with a range of 100-1 100 m/z.Results 154 components were identified at one time,of which 28 were positive and negative ion modes.Conclusion The composition of Fuzheng Huayu capsule was comprehensively clarified in this study,which laid a foundation for further research of this compound.
keywords:Fuzheng Huayu capsule  identification  chemical composition
查看全文  查看/发表评论  下载PDF阅读器
关闭

分享按钮