复方黄连灌肠液的总黄酮测定方法研究
投稿时间:2019-01-03  修订日期:2019-05-18  点此下载全文
引用本文:倪晓霞,王庆芬,刘晓玲,叶财发.复方黄连灌肠液的总黄酮测定方法研究[J].药学实践杂志,2019,37(4):352~356,374
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作者单位
倪晓霞 中国人民解放军联勤保障部队第九〇九医院/厦门大学附属东南医院制剂科, 福建 漳州 363000 
王庆芬 中国人民解放军联勤保障部队第九〇九医院/厦门大学附属东南医院制剂科, 福建 漳州 363000 
刘晓玲 中国人民解放军联勤保障部队第九〇九医院/厦门大学附属东南医院制剂科, 福建 漳州 363000 
叶财发 中国人民解放军联勤保障部队第九〇九医院/厦门大学附属东南医院制剂科, 福建 漳州 363000 
基金项目:解放军第175医院青年苗圃课题项目(NO.16Y025)
中文摘要:目的 建立复方黄连灌肠液中总黄酮含量测定方法。方法 采用紫外-可见分光光度法测定。比较直接测定、AlCl3-CH3COONa显色体系、NaNO2-Al (NO33-NaOH显色体系等3种不同显色方法,分析以槲皮素、芦丁、橙皮苷为对照品的波长扫描图谱,探讨最佳测定条件。结果 最佳显色方案为采用NaNO2-Al (NO33-NaOH显色法,以芦丁为对照品,在371 nm波长下进行测定;芦丁在2.488~12.44 μg/ml范围内线性关系良好(r=0.9995),加样回收率平均值为100.43%(RSD=1.33%)。结论 NaNO2-Al (NO33-NaOH显色法适用于复方黄连灌肠液总黄酮的含量测定,该法简便、快速、稳定可靠。
中文关键词:复方黄连灌肠液  紫外-可见分光光度法  总黄酮  含量测定
 
Determination for total flavonoids in compound Huanglian clysis
Abstract:Objective To establish a method for the determination of the content of total flavonoids in compound Huanglian clysis.Methods Three reference substances and the three coloration methods were studied to find the ideal coloration method and determination conditions by spectrophotometry.Results The sample was detected at 371nm wavelength by NaNO2-Al(NO3)3-NaOH reaction with rutin as reference substance.A good linear relationship was observed in the concentration range of 2.488~12.44μg/ml for querceetin (r=0.9993),and the average recovery rate was 100.43% with RSD of 1.33%.Conclusion The NaNO2-Al(NO3)3-NaOH reaction was simple,quick,stable and reliable,which was proved to be applicable for the determination of the content of total flavonoids in compound Huanglian clysis.
keywords:Compound Huanglian clysis  UV spectrophotometry  Total flavonoids  Content determination
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