月腺大戟的化学成分及其乳腺癌细胞毒活性研究
投稿时间:2018-04-25  修订日期:2019-02-25  点此下载全文
引用本文:曹青青,李盛建,李云青,葛继云,陈俊,周瑾,钱跹,赵亮.月腺大戟的化学成分及其乳腺癌细胞毒活性研究[J].药学实践杂志,2019,37(4):309~313,317
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作者单位E-mail
曹青青 上海市浦东新区人民医院药剂科, 上海 201299  
李盛建 上海东方肝胆外科医院药材科, 上海 200438  
李云青 山西省儿童医院药剂科, 山西 太原 030013  
葛继云 上海东方肝胆外科医院药材科, 上海 200438  
陈俊 上海东方肝胆外科医院药材科, 上海 200438  
周瑾 上海市宝山区罗店医院药剂科, 上海 201908  
钱跹 上海东方肝胆外科医院药材科, 上海 200438  
赵亮 上海市宝山区罗店医院药剂科, 上海 201908 zhaoliangphar@163.com 
基金项目:国家自然科学基金—青年项目(81303300)
中文摘要:目的 研究大戟科植物月腺大戟的化学成分及其对乳腺癌细胞毒活性。方法 采用多种色谱技术对化合物进行分离纯化,根据化合物的理化性质和光谱数据分析鉴定化合物的结构。采用噻唑蓝(MTT)比色法研究化合物对6种乳腺癌细胞的抑制活性。结果 月腺大戟植物中分离得到10个化合物,分别鉴定为:2,4-二羟基-6-甲氧基-3-甲酰基苯乙酮(1);2,4-二羟基-6-甲氧基-3-甲基苯乙酮(2);月腺大戟素A(3);3,3'-乙酰基-4,4'-二甲氧基-2,2',6,6'-四羟基二苯基甲烷(4);岩大戟内酯B(5);岩大戟内酯A(6);亚油酸(7);fischeria A(8);β-香树脂醇乙酸酯(9);单十九酸甘油酯(10)。乳腺癌细胞毒活性研究显示:化合物3对乳腺癌细胞MDA-MB-231、Sum149、MCF7、ZR-75-1、SKBr3、BT474的IC50值分别为6.69、5.50、5.50、7.08、8.64、5.42 μmol/L。结论 化合物6、7、8、10为首次从该植物中分离得到;化合物3对乳腺癌细胞具有显著的抑制活性。
中文关键词:月腺大戟  化学成分  结构鉴别  乳腺癌  细胞毒活性
 
Chemical constituents and cytotoxic activities in breast cancer of Euphorbia ebracteolata
Abstract:Objective To investigate the chemical constituents and cytotoxic activities of breast cancer in the roots of Euphorbia ebracteolata.Methods The chemical constituents were isolated and purified by various chromatographic techniques.The in vitro cytotoxic activities of breast cancer of the isolated compounds were studied by MTT method.Their structures were identified on the basis of spectroscopic analyses and physiochemical properties.Results Ten compounds were isolated from the roots of Euphorbia ebracteolata,and elucidated as 2,4-dihydroxy-6-methoxy-3-form yl-1-acetophenon (1),2,4-dihydroxy-6-methoxy- 3-methyl-1- acetophenon (2),ebracteolatain A (3),3,3'-diacetyl-4,4'-dimethoxy-2,2',6,6'-tetrahydroxy diphenylmethan (4),jolkinolide B (5),jolkinolide A (6),linoleic acid (7),fischeria A (8),β-amyrin acetate (9),nonadecanoic acid monoglyceride (10).Compound 3 showed cytotoxicity against MDA-MB-231 cell、Sum149 cell、MCF7 cell、ZR-75-1 cell、SKBr3 cell、BT474 cell with IC50values of (6.69±0.128),(5.50±0.126),(5.50±0.126),(7.08±0.111),(8.64±0.197),(5.42±0.113)μmol/L,respectively.Compound 4 had no cytotoxicity on the above cancer cells.Conclusion Compounds 6,7,8,10 were firstly isolated from Euphorbia ebracteolata.Compound 3 showed significant cytotoxicity on breast cancer cells.
keywords:Euphorbia ebracteolata  chemical constituents  structure identified  breast cancer  cytotoxic activities
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